Echo 650T Liquid Handler

The New Sound of Sample Management

The Echo 650T Liquid Handler supports sample transfer directly from Echo Qualified sample tubes, enabling a fully non-contact liquid handling workflow from sample storage all the way to assay ready plates. Capable of transferring as little as 2.5 nL from 384-well and 1536-well microplates, and now, from sample tubes arrayed in a 96-tube format, the Echo 650T Liquid Handler meets the needs of a wide variety of molecular and cellular assays.

Key Benefits

Upgrade to increase transfer speed for high-throughput applications
Simplified sample screening through direct transfer from Echo Qualified sample tubes
Accurate, precise movement of nanoliter-scale quantities, enabling assay miniaturization, further reducing costs
Simplified assay development and dilution processes through the flexibility to transfer any volume of liquid from any well to any other well
Better results through direct dilution of compounds, an improvement over serial dilution method

Revolutionizing Sample Managementwith an All Acoustic Workflow

from sample store to assay-ready microplates

With diverse fluid transfer capabilities and high precision and accuracy, the Echo 650T Liquid Handler supports a wide range of applications in drug discovery and basic research.

for DRUG DISCOVERY

Acoustic Sample Tube Management

The FluidX AcoustiX Sample Tube (FAST) was developed in collaboration with and is sold by Brooks Life Sciences for ultra-high-density sample storage. The acoustic tube offers the following benefits:

Echo Qualified design - compatible with Echo 650T and 655T Liquid Handlers
Preserves sample integrity from storage to screen
Miniaturize upstream liquid handling steps to conserve sample
Rapidly pick sets of samples for targeted screening

Workflow Automation

To meet the broad spectrum of sample throughput needs, the Echo 650T Liquid Handler seamlessly integrates with the highly configurable Access™ Dual Robot System accommodating a wide variety of devices and enabling a fully automated workflow.

Configured to achieve optimal throughput for sample management workflows
Compact and ergonomic design with docking modules, turntables, retractable shelves, and drawers
Optional features can include environmental management to protect samples from hydration or evaporation

Echo Acoustic Liquid Handling|by Labcyte Inc.

This video shows the Echo acoustic droplet ejection (ADE) technology in action. Sound waves eject precisely-sized droplets from the source plate onto the destination plate suspended above.

Impacting Therapeutic Discovery

Tipless, non-contact, acoustic fluid transfers eliminate the potential for samples or compounds to adhere to plastic tips. This helps to improve data quality, increasing the potential to find new therapeutics.

In a screen of 975 compounds, Bristol-Myers Squibb found 108 more hits when using the Echo Liquid Handler¹.

¹ Spicer T., Fitzgerald Y., Burford N., Matson S., Chatterjee M., Gilchrist M.,Myslik J., O'Connell J.
Pharmacological evaluation of different compound dilution and transfer paradigms on an enzyme assay in low volume 384-well format. Poster presented at Drug Discovery Technology, August 2005, Boston, MA.

32% More Active Compounds Found

Potent by

Echo transfer

108 | 32%

Potent by

both methods

230 | 68%

Save Compounds and Money by Miniaturizing Assays

Set up 384-well cell-based and biochemical assays or transition into 1536-well formats with no loss of performance. The nanoliter volume transfers of the Echo 655T systems enable direct dilution of concentrated compounds from sample tubes – eliminating workflow steps and keeping the final concentration of DMSO low in the final assay. Data accuracy is further improved through the elimination of compound loss on tips.

Decrease Cost and Maintain Data Quality

The high-performance fluid transfer of Echo 650T Liquid Handler delivers comparable transfer efficiency, knock-down, and cell morphology reactions to traditional liquid handlers with less use of sample and reagent.

FIGURE 1: Fluorescence image reveals KYSE cells successfully transfected with siGLO(GE Healthcare) (pink), transferred using the Echo Liquid Handler¹

Sample

■ Echo Dispensed
■ Manually Dispensed

FIGURE 2: Inclusion intensities of esophageal cancer cells transfected using the Echo Liquid Handler vs. manual transfer. Error bars indicate standard deviations.¹

¹Jarman C., Edwards C., Bennett E., Bryne A., Hoff A., Bakos G., Kelleher D., Long A., Davies A. Miniaturizing siRNA Transfection Using the Echo Liquid Handler. Labcyte Application Note.

Transition Into High-Throughput Formats

High assay performance at miniaturized volumes enables researchers to move quantitative assays into higher density formats. Assays such as time-dependent inhibition perform comparably to traditional volumes with less reagent, compound, and the elimination of workflow steps.

1
2
3
4
5

- NADPH
+ NADPH

FIGURE 3: Time-dependent CYP3A4 inhibition profiling with two inhibitors.²

Description	EC₅₀ (nM)
- NADPH	19.14
+ NADPH	18.26
δ IC₅₀	1

1
2
3
4
5

- NADPH
+ NADPH

FIGURE 2: Time-dependent CYP1A2 inhibition profiling with two inhibitors

Description	EC₅₀ (nM)
- NADPH	11224
+ NADPH	258.6
δ IC₅₀	43

²Wang J. Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo 555 Liquid Handler. Labcyte Application Note.

Echo 650T Specifications

Description	Specifications
Drop volume	2.5 nL
Volume transfer range	2.5 nL – 5 µL (dependent on fluid type, assay, and source plate used)
Reagents supported	DMSO, PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, antibodies, enzymes, and protein crystallography reagents
Transfer accuracy	<10% deviation from target volume
Transfer precision	<8% CV (except protein crystallography fluid class)
Source labware compatibility	All Echo Qualified 384- and 1536-well Microplates and Echo Qualified acoustic sample tubes
Destination labware compatibility	All Echo Qualified Microplates and most ANSI-compliant/SBS-standard microplates in 96-, 384-, 1536-, and 3456-well formats 8 to 16 mm well height
Barcode reader options	Short-side and/or long-side
Dimensions	53.9 cm width x 68.3 cm depth x 92.5 cm height (21.2 x 26.9 x 36.4 inches)
Weight	130 kg (286 lbs)
Working envelope	Additional 25.4 cm (10 inches) on top, 2.5 cm (1 inch) on sides, 7.6 cm (3 inches) at front, 30.5 cm (12 inches) at back
Electrical	AC 120V, 50/60 Hz, 10 A or AC 230V, 50/60Hz, 5 A
Operating temperature	21°C ± 5°C (70°F ± 9°F)
Vacuum	House supply minimum 200 Torr (266 mbar, 22 inches Hg Vac) OR Standalone vacuum with surge tank, minimum pump speed 2.0 m³/hr, minimum pressure 100 Torr (133 mbar, 26 inches Hg Vac)
Fluid class calibration	No calibration needed