Echo 655T Liquid Handler

The New Sound of Sample Management

The Echo 655T Liquid Handler supports sample transfer directly from Echo Qualified sample tubes, enabling a fully non-contact liquid handling workflow from sample storage all the way to assay ready plates. The Echo 655T Liquid Handler is capable of transferring as little as 2.5 nL from 384-well and 1536-well microplates, and now, from sample tubes arrayed in a 96-tube format. Additionally, it transfers at the highest speed to meet the needs of high-thoughput biochemical and cell-based screening.

Key Benefits

Fastest dispense rate from an Echo Qualified sample tube
Simplified compound library screening through direct transfer from acoustic sample tubes
Elimination of compound loss, cross-contamination, carryover, or leachates through non-contact transfer
Minimized DMSO hydration through environment controlled transfer zone

Revolutionizing Sample Managementwith an All Acoustic Workflow

from sample store to assay-ready microplates

With a combination of high transfer speeds and diverse fluid transfer capabilities, the Echo 655T Liquid Handler supports a wide range of applications in drug discovery and basic research.

for DRUG DISCOVERY

Acoustic Sample Tube Management

The FluidX AcoustiX Sample Tube (FAST) was developed in collaboration with and is sold by Brooks Life Sciences for ultra-high-density sample storage. The acoustic tube offers the following benefits:

Echo Qualified design - compatible with Echo 650T and 655T Liquid Handlers
Preserves sample integrity from storage to screen
Miniaturize upstream liquid handling steps to conserve sample
Rapidly pick sets of samples for targeted screening

Workflow Automation

To meet the broad spectrum of sample throughput needs, the Echo 655T Liquid Handler seamlessly integrates with the highly configurable Access™ Dual Robot System accommodating a wide variety of devices and enabling a fully automated workflow.

Configured to achieve optimal throughput for sample management workflows
Compact and ergonomic design with docking modules, turntables, retractable shelves, and drawers
Optional features can include environmental management to protect samples from hydration or evaporation

Echo Acoustic Liquid Handling|by Labcyte Inc.

Watch the Echo acoustic droplet ejection (ADE) technology in action. Sound waves eject precisely-sized droplets from the source plate onto the destination plate suspended above.

Impacting Therapeutic Discovery

Tipless, non-contact, acoustic fluid transfers eliminate the potential for samples or compounds to adhere to plastic tips. This helps to improve data quality, increasing the potential to find new therapeutics.

In a screen of 975 compounds, Bristol-Myers Squibb found 108 more hits when using the Echo Liquid Handler¹.

¹ Spicer T., Fitzgerald Y., Burford N., Matson S., Chatterjee M., Gilchrist M.,Myslik J., O'Connell J.
Pharmacological evaluation of different compound dilution and transfer paradigms on an enzyme assay in low volume 384-well format. Poster presented at Drug Discovery Technology, August 2005, Boston, MA.

32% More Active Compounds Found

Potent by

Echo transfer

108 | 32%

Potent by

both methods

230 | 68%

Save Compounds and Money by Miniaturizing Assays

Set up 384-well cell-based and biochemical assays or transition into 1536-well formats with no loss of performance. The nanoliter volume transfers of the Echo 655T systems enable direct dilution of concentrated compounds from sample tubes – eliminating workflow steps and keeping the final concentration of DMSO low in the final assay. Data accuracy is further improved through the elimination of compound loss on tips.

FIGURE 1: Stimulation curves and EC₅₀ values for isoproterenol, salbutamol, and salmeterol in 384-well (left) and 1536-well (right), LV white microplate. Incubation time of 60 minutes. BMG Labtech PHERAstar FS.¹

● Isoprotenol
■ Salbutamol
Salmeterol

FIGURE 1: Stimulation curves and EC₅₀ values for isoproterenol, salbutamol, and salmeterol in 384-well, LV white microplate. Incubation time of 60 minutes. BMG Labtech PHERAstar FS.¹

Description	EC₅₀
Isoproterenol	0.8 nM
Salbutamol	11 nM
Salmeterol	3.2 nM
Z’ Factor	0.72

● Isoprotenol
■ Salbutamol
Salmeterol

FIGURE 2: Stimulation curves and EC₅₀ values for isoproterenol, salbutamol, and salmeterol in 1536-well, LV white microplate. Incubation time of 60 minutes. BMG Labtech PHERAstar FS.¹

Description	EC₅₀
Isoproterenol	0.8 nM
Salbutamol	27 nM
Salmeterol	0.2 nM
Z’ Factor	0.89

¹Edwards B., Tang N., Nakamura M. Miniaturized HTRF Assays Using the Echo Liquid Handler. Labcyte Application Note.

Making High-throughput Combination Screening a Reality

Drug combination screening using the Echo system has revolutionized the approach to the assessment of synergistic effects of drug combinations on disease. The capability to transfer from any-well-to-any-well and the Echo Combination Screening Software make high throughput, 2-way and 3-way dose response matrix studies a reality.

Echo 655T Specifications

Description	Specifications
Drop volume	2.5 nL
Volume transfer range	2.5 nL – 5 µL (dependent on fluid type, assay, and source plate used)
Reagents supported	DMSO, PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, antibodies, enzymes, and protein crystallography reagents
Transfer accuracy	<10% deviation from target volume
Transfer precision	<8% CV (except protein crystallography fluid class)
Source labware compatibility	All Echo Qualified 384- and 1536-well Microplates and Echo Qualified acoustic sample tubes
Destination labware compatibility	All Echo Qualified Microplates and most ANSI-compliant/SBS-standard microplates in 96-, 384-, 1536-, and 3456-well formats 8 to 16 mm well height
Barcode reader options	Short-side and/or long-side
Dimensions	53.9 cm width x 68.3 cm depth x 92.5 cm height (21.2 x 26.9 x 36.4 inches)
Weight	130 kg (286 lbs)
Working envelope	Additional 25.4 cm (10 inches) on top, 2.5 cm (1 inch) on sides, 7.6 cm (3 inches) at front, 30.5 cm (12 inches) at back
Electrical	AC 120V, 50/60 Hz, 10 A or AC 230V, 50/60Hz, 5 A
Operating temperature	21°C ± 5°C (70°F ± 9°F)
Vacuum	House supply minimum 200 Torr (266 mbar, 22 inches Hg Vac) OR Standalone vacuum with surge tank, minimum pump speed 2.0 m³/hr, minimum pressure 100 Torr (133 mbar, 26 inches Hg Vac)
Fluid class calibration	No calibration needed