Echo 650 Liquid Handler

Another Step Forward for Acoustic Liquid Handling

The Echo 650 Liquid Handler is ideal for medium-throughput applications. It enables the transfer of as little as 2.5 nL from 384-well and 1536-well microplates. As your research needs change, upgrade packages are available that enable the addition of Echo Qualified sample tube capabilities.

Key Benefits

Measure DMSO hydration levels to gain insights into sample integrity and improve transfer quality
Elimination of compound loss, cross-contamination, carryover, or leachates through non-contact transfer
Accurate and precise transfer of nanoliter-scale quantities, enabling assay miniaturization, further reducing costs
Better results through direct dilution of compounds, an improvement over serial dilution methods
Flexibility to transfer any volume of liquid from any source well to any destination well

A Broad Range of Applications

With diverse fluid transfer capabilities and high precision and accuracy, the Echo 650 Liquid Handler supports a wide range of applications in drug discovery and basic research.

for DRUG DISCOVERY

Echo Acoustic Liquid Handling|by Labcyte Inc.

Watch the Echo acoustic droplet ejection (ADE) technology in action. Sound waves eject precisely-sized droplets from the source plate onto the destination plate suspended above.

Impacting Therapeutic Discovery

Tipless, non-contact, acoustic fluid transfers eliminate the potential for samples or compounds to adhere to plastic tips. This helps to improve data quality, increasing the potential to find new therapeutics.

In a screen of 975 compounds, Bristol-Myers Squibb found 108 more hits when using the Echo Liquid Handler¹.

¹ Spicer T., Fitzgerald Y., Burford N., Matson S., Chatterjee M., Gilchrist M.,Myslik J., O'Connell J.
Pharmacological evaluation of different compound dilution and transfer paradigms on an enzyme assay in low volume 384-well format. Poster presented at Drug Discovery Technology, August 2005, Boston, MA.

32% More Active Compounds Found

Potent by

Echo transfer

108 | 32%

Potent by

both methods

230 | 68%

Save Compounds and Money by Miniaturizing Assays

Set up 384-well cell-based and biochemical assays or transition into 1536-well formats with no loss of performance. The nanoliter volume transfers of the Echo 655T systems enable direct dilution of concentrated compounds from sample plates – eliminating workflow steps and keeping the final concentration of DMSO low in the final assay. Data accuracy is further improved through the elimination of compound loss on tips.

Decrease Cost and Maintain Data Quality

The high-performance fluid transfer of Echo 650 Liquid Handler delivers comparable transfer efficiency, knock-down, and cell morphology reactions to traditional liquid handlers with less use of sample and reagent.

FIGURE 1: Fluorescence image reveals KYSE cells successfully transfected with siGLO(GE Healthcare) (pink), transferred using the Echo Liquid Handler¹

Sample

■ Echo Dispensed
■ Manually Dispensed

FIGURE 2: Inclusion intensities of esophageal cancer cells transfected using the Echo Liquid Handler vs. manual transfer. Error bars indicate standard deviations.¹

¹Jarman C., Edwards C., Bennett E., Bryne A., Hoff A., Bakos G., Kelleher D., Long A., Davies A. Miniaturizing siRNA Transfection Using the Echo Liquid Handler. Labcyte Application Note.

Transition Into High-Throughput Formats

High assay performance at miniaturized volumes enables researchers to move quantitative assays into higher density formats. Assays such as time-dependent inhibition perform comparably to traditional volumes with less reagent, compound, and the elimination of workflow steps.

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2
3
4
5

- NADPH
+ NADPH

FIGURE 3: Time-dependent CYP3A4 inhibition profiling with two inhibitors.²

Description	EC₅₀ (nM)
- NADPH	19.14
+ NADPH	18.26
δ IC₅₀	1

1
2
3
4
5

- NADPH
+ NADPH

FIGURE 4: Time-dependent CYP1A2 inhibition profiling with two inhibitors²

Description	EC₅₀ (nM)
- NADPH	11224
+ NADPH	258.6
δ IC₅₀	43

²Wang J. Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo 555 Liquid Handler. Labcyte Application Note.

Echo 650 Specifications

Description	Specifications
Drop volume	2.5 nL
Volume transfer range	2.5 nL – 5 µL (dependent on fluid type, assay, and source plate used)
Reagents supported	DMSO, PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, antibodies, enzymes, and protein crystallography reagents
Transfer accuracy	<10% deviation from target volume
Transfer precision	<8% CV (except protein crystallography fluid class)
Source labware compatibility	All Echo Qualified 384- and 1536-well Microplates
Destination labware compatibility	All Echo Qualified Microplates and most ANSI-compliant/SBS-standard microplates in 96-, 384-, 1536-, and 3456-well formats 8 to 16 mm well height
Barcode reader options	Short-side and/or long-side
Dimensions	53.9 cm width x 68.3 cm depth x 92.5 cm height (21.2 x 26.9 x 36.4 inches)
Weight	130 kg (286 lbs)
Working envelope	Additional 25.4 cm (10 inches) on top, 2.5 cm (1 inch) on sides, 7.6 cm (3 inches) at front, 30.5 cm (12 inches) at back
Electrical	AC 120V, 50/60 Hz, 10 A or AC 230V, 50/60Hz, 5 A
Operating temperature	21°C ± 5°C (70°F ± 9°F)
Vacuum	House supply minimum 200 Torr (266 mbar, 22 inches Hg Vac) OR Standalone vacuum with surge tank, minimum pump speed 2.0 m³/hr, minimum pressure 100 Torr (133 mbar, 26 inches Hg Vac)
Fluid class calibration	No calibration needed