Echo 655 Liquid Handler

Another Step Forward for Acoustic Liquid Handling

The Echo 655 Liquid Handler supports the highest speed transfer of as little as 2.5 nL from 384-well and 1536-well microplates to meet the needs of high-thoughput biochemical and cell-based screening. As your research needs change, upgrade packages are available that enable the addition of Echo Qualified sample tube capabilities.

Key Benefits

Fastest dispense rate for acoustic liquid handling
Measure DMSO hydration levels to gain insights into sample integrity and improve transfer quality
Elimination of compound loss, cross-contamination, carryover, or leachates through non-contact transfer
Extend compound library life through miniaturization of assays
Flexibility to transfer any volume of liquid from any source well to any destination well

A Broad Range of Applications

With a combination of high transfer speeds and diverse fluid transfer capabilities, the Echo 655 Liquid Handler supports a wide range of applications in drug discovery and basic research.

for DRUG DISCOVERY

Echo Acoustic Liquid Handling|by Labcyte Inc.

Watch the Echo acoustic droplet ejection (ADE) technology in action. Sound waves eject precisely-sized droplets from the source plate onto the destination plate suspended above.

Impacting Therapeutic Discovery

Tipless, non-contact, acoustic fluid transfer of the Echo 655 Liquid Handler eliminates the potential for samples or compounds to adhere to tips. This helps to improve data quality, increasing the potential to find new therapeutics.

In a screen of 975 compounds, Bristol-Myers Squibb found 108 more hits when using the Echo Liquid Handler¹.

¹ Spicer T., Fitzgerald Y., Burford N., Matson S., Chatterjee M., Gilchrist M.,Myslik J., O'Connell J.
Pharmacological evaluation of different compound dilution and transfer paradigms on an enzyme assay in low volume 384-well format. Poster presented at Drug Discovery Technology, August 2005, Boston, MA.

32% More Active Compounds Found

Potent by

Echo transfer

108 | 32%

Potent by

both methods

230 | 68%

Save Compounds and Money by Miniaturizing Assays

Set up 384-well cell-based and biochemical assays or transition into 1536-well formats with no loss of performance. The nanoliter volume transfers of the Echo 655 system enables direct dilution of concentrated compounds from sample plates – eliminating workflow steps and keeping the final concentration of DMSO low in the final assay. Data accuracy is further improved through the elimination of compound loss on tips.

FIGURE 1: Stimulation curves and EC₅₀ values for isoproterenol, salbutamol, and salmeterol in 384-well (left) and 1536-well (right), LV white microplate. Incubation time of 60 minutes. BMG Labtech PHERAstar FS.¹

● Isoprotenol
■ Salbutamol
Salmeterol

FIGURE 1: Stimulation curves and EC₅₀ values for isoproterenol, salbutamol, and salmeterol in 384-well, LV white microplate. Incubation time of 60 minutes. BMG Labtech PHERAstar FS.¹

Description	EC₅₀
Isoproterenol	0.8 nM
Salbutamol	11 nM
Salmeterol	3.2 nM
Z’ Factor	0.72

● Isoprotenol
■ Salbutamol
Salmeterol

FIGURE 2: Stimulation curves and EC₅₀ values for isoproterenol, salbutamol, and salmeterol in 1536-well, LV white microplate. Incubation time of 60 minutes. BMG Labtech PHERAstar FS.¹

Description	EC₅₀
Isoproterenol	0.8 nM
Salbutamol	27 nM
Salmeterol	0.2 nM
Z’ Factor	0.89

¹Edwards B., Tang N., Nakamura M. Miniaturized HTRF Assays Using the Echo Liquid Handler. Labcyte Application Note.

Echo 655 Specifications

Description	Specifications
Drop volume	2.5 nL
Volume transfer range	2.5 nL – 5 µL (dependent on fluid type, assay, and source plate used)
Reagents supported	DMSO, PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, antibodies, enzymes, and protein crystallography reagents
Transfer accuracy	<10% deviation from target volume
Transfer precision	<8% CV (except protein crystallography fluid class)
Source labware compatibility	All Echo Qualified 384- and 1536-well Microplates
Destination labware compatibility	All Echo Qualified Microplates and most ANSI-compliant/SBS-standard microplates in 96-, 384-, 1536-, and 3456-well formats 8 to 16 mm well height
Barcode reader options	Short-side and/or long-side
Dimensions	53.9 cm width x 68.3 cm depth x 92.5 cm height (21.2 x 26.9 x 36.4 inches)
Weight	130 kg (286 lbs)
Working envelope	Additional 25.4 cm (10 inches) on top, 2.5 cm (1 inch) on sides, 7.6 cm (3 inches) at front, 30.5 cm (12 inches) at back
Electrical	AC 120V, 50/60 Hz, 10 A or AC 230V, 50/60Hz, 5 A
Operating temperature	21°C ± 5°C (70°F ± 9°F)
Vacuum	House supply minimum 200 Torr (266 mbar, 22 inches Hg Vac) OR Standalone vacuum with surge tank, minimum pump speed 2.0 m³/hr, minimum pressure 100 Torr (133 mbar, 26 inches Hg Vac)
Fluid class calibration	No calibration needed