Echo 550 Liquid Handler

Impacting Science with Sound

Acoustic liquid handling is changing how researchers are doing science. The precise nL transfer volumes, the any-well-to-any-well transfer capability, and flexible applications software of the Echo 550 Liquid Handler enables researchers to set up experiments not possible with traditional liquid handling.

Key Benefits

Better information about compound integrity and improved transfer quality through measurement of DMSO hydration level
Eliminates sample and compound loss, cross-contamination, carryover or leachates
Accurate, precise transfer of nanoliter-scale increments, enabling assay miniaturization, further reducing costs
Better results through direct dilution of compounds
Simplified assay development and dilution processes through the flexibility to transfer any volume of liquid from any well to any other well

A Broad Range of Applications

With a combination of high transfer speeds and diverse fluid transfer capabilities, the Echo 550 Liquid Handler supports a wide range of applications in drug discovery and basic research.

for GENOMICS

SANGER SEQUENCING ASSAY SET-UP

for DRUG DISCOVERY

HIGH-THROUGHPUT SCREENING
HIGH CONTENT SCREENING
ASSAY-READY PLATES
EPIGENETICS
IC₅₀

Echo Acoustic Liquid Handling|by Labcyte Inc.

Watch the Echo acoustic droplet ejection (ADE) technology in action. Sound waves eject precisely-sized droplets from the source plate onto the destination plate suspended above.

Decrease Cost and Maintain Data Quality

The high-performance fluid transfer of Echo 550 Liquid Handler delivers comparable transfer efficiency, knock-down, and cell morphology reactions to traditional liquid handlers with less use of sample and reagent.

FIGURE 1: Fluorescence image reveals KYSE cells successfully transfected with siGLO(GE Healthcare) (pink), transferred using the Echo Liquid Handler¹

Sample

■ Echo Dispensed
■ Manually Dispensed

FIGURE 2: Inclusion intensities of esophageal cancer cells transfected using the Echo Liquid Handler vs. manual transfer. Error bars indicate standard deviations.¹

¹Jarman C., Edwards C., Bennett E., Bryne A., Hoff A., Bakos G., Kelleher D., Long A., Davies A. Miniaturizing siRNA Transfection Using the Echo Liquid Handler. Labcyte Application Note.

Transition Into High-Throughput Formats

High assay performance at miniaturized volumes enables researchers to move quantitative assays into higher density formats. Assays such as time-dependent inhibition perform comparably to traditional volumes with less reagent, compound, and the elimination of workflow steps.

1
2
3
4
5

- NADPH
+ NADPH

FIGURE 3: Time-dependent CYP3A4 inhibition profiling with two inhibitors.²

	EC₅₀ (nM)
- NADPH	40.27
+ NADPH	7.96
δ IC₅₀	5

1
2
3
4
5

- NADPH
+ NADPH

FIGURE 4: Time-dependent CYP1A2 inhibition profiling with two inhibitors²

	EC₅₀ (nM)
- NADPH	14273
+ NADPH	150.3
δ IC₅₀	95

²Wang J. Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo 555 Liquid Handler. Labcyte Application Note.

Echo 550 Specifications

Description	Specifications
Drop volume	2.5 nL
Volume transfer range	2.5 nL – 10 µL (dependent on fluid type, assay, and source plate used)
Reagents supported	DMSO, PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, antibodies, enzymes, and protein crystallography reagents
Transfer accuracy	<10% deviation from target volume
Transfer precision	<8% CV (except protein crystallography fluid class)
Source labware compatibility	All Echo Qualified 384- and 1536-well Microplates
Destination labware compatibility	All Echo Qualified Microplates and most ANSI-compliant/SBS-standard microplates in 96-, 384-, 1536-, and 3456-well formats 8 to 16 mm well height
Barcode reader options	Short-side and/or long-side
Dimensions	53.9 cm width x 68.3 cm depth x 92.5 cm height (21.2 x 26.9 x 36.4 inches)
Weight	128 kg (283 lbs)
Working envelope	Additional 25.4 cm (10 inches) on top, 2.5 cm (1 inch) on sides, 7.6 cm (3 inches) at front, 30.5 cm (12 inches) at back
Electrical	AC 120V, 50/60 Hz, 10 A or AC 230V, 50/60Hz, 5 A
Operating temperature	21°C ± 5°C (70°F ± 9°F)
Vacuum	House supply minimum 200 Torr (266 mbar, 22 inches Hg Vac) OR Standalone vacuum with surge tank, minimum pump speed 2.0 m³/hr, minimum pressure 100 Torr (133 mbar, 26 inches Hg Vac)
Fluid class calibration	No calibration needed