for high throughput screening
| Description | Specifications |
|---|---|
| Drop volume | 2.5 nL |
| Volume transfer range | 2.5 nL – 10 µL (dependent on fluid type, assay, and source plate used) |
| Reagents supported | DMSO, PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, antibodies, enzymes, and protein crystallography reagents |
| Transfer accuracy | <10% deviation from target volume |
| Transfer precision | <8% CV (except protein crystallography fluid class) |
| Source labware compatibility | All Echo Qualified 384- and 1536-well Microplates |
| Destination labware compatibility | All Echo Qualified Microplates and most ANSI-compliant/SBS-standard microplates in 96-, 384-, 1536-, and 3456-well formats 8 to 16 mm well height |
| Barcode reader options | Short-side and/or long-side |
| Dimensions | 53.9 cm width x 68.3 cm depth x 92.5 cm height (21.2 x 26.9 x 36.4 inches) |
| Weight | 128 kg (283 lbs) |
| Working envelope | Additional 25.4 cm (10 inches) on top, 2.5 cm (1 inch) on sides, 7.6 cm (3 inches) at front, 30.5 cm (12 inches) at back |
| Electrical | AC 120V, 50/60 Hz, 10 A or AC 230V, 50/60Hz, 5 A |
| Operating temperature | 21°C ± 5°C (70°F ± 9°F) |
| Vacuum | House supply minimum 200 Torr (266 mbar, 22 inches Hg Vac) OR Standalone vacuum with surge tank, minimum pump speed 2.0 m3/hr, minimum pressure 100 Torr (133 mbar, 26 inches Hg Vac) |
| Fluid class calibration | No calibration needed |
The Echo 555 Liquid Handler has the speed and flexibility required for a screening lab. With rapid, highly precise nL volume transfers, Echo 555 Liquid Handler can process over 650* compound and over 500* reagent plates per day. Tasks like plate reformatting, hit picking, daughter plate creation, and adding samples and reagents for screening assays are made easy with the Echo 555 Liquid Handler. *Based on 100 nL volume transfers
Going beyond DMSO used in high throughput screening, the Echo 555 system supports the transfer of a broad range of aqueous solutions to support high throughput screening laboratories.
VIDEO
The Echo Liquid Handler revolutionizes liquid handling with acoustic energy. Sound waves eject precisely sized droplets from a source onto a microplate, slide or other surface suspended above the source.
Tipless, non-contact, acoustic fluid transfer of the Echo 555 Liquid Handler eliminates the potential for samples or compounds to adhere to tips. This helps to improve data quality, increasing the potential to find new therapeutics.
Potent by
Echo transfer
108 | 32%
Potent by
both methods
230 | 68%
In a screen of 975 compounds, Bristol-Myers Squibb found 108 more hits when using the Echo Liquid Handler1.
1 Spicer T., Fitzgerald Y., Burford N., Matson S., Chatterjee M., Gilchrist M.,Myslik J., O'Connell J.
Pharmacological evaluation of different compound dilution and transfer paradigms on an enzyme assay in low volume 384-well format. Poster presented at Drug Discovery Technology, August 2005, Boston, MA.
Utilize the Echo 555 Liquid Handler to set up 384-well cell-based and biochemical assays or transition into 1536-well formats with no loss of performance. The nL volume transfers of the Echo 555 systems enable direct dilution of concentrated compounds – eliminating workflow steps and keeping the final concentration of DMSO low in the final assay. Data accuracy is further improved through the elimination of compound loss on tips.
FIGURE 1: Stimulation curves and EC50 values for isoproterenol, salbutamol, and salmeterol in 384-well (left) and 1536-well (right), LV white microplate. Incubation time of 60 minutes. BMG Labtech PHERAstar FS.
FIGURE 1: Stimulation curves and EC50 values for isoproterenol, salbutamol, and salmeterol in 384-well, LV white microplate. Incubation time of 60 minutes. BMG Labtech PHERAstar FS.
| EC50 | |
|---|---|
| Isoproterenol | 0.8 nM |
| Salbutamol | 11 nM |
| Salmeterol | 3.2 nM |
| Z’ Factor | 0.72 |
FIGURE 2: Stimulation curves and EC50 values for isoproterenol, salbutamol, and salmeterol in 1536-well, LV white microplate. Incubation time of 60 minutes. BMG Labtech PHERAstar FS.
| EC50 | |
|---|---|
| Isoproterenol | 0.8 nM |
| Salbutamol | 27 nM |
| Salmeterol | 0.2 nM |
| Z’ Factor | 0.89 |
Drug combination screening using the Echo 555 Liquid Handler has revolutionized the approach to the assessment of synergistic effects of drug combinations on disease. The any-well-to-any-well fluid transfer and the Echo Combination Screening application software makes high throughput, 2-way and 3-way dose response matrix studies a reality.
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