Echo 550 LIQUID HANDLER

for medium throughput screening and large genomics projects

  • Most diverse application range of the Echo Liquid Handler family
  • Impact all phases of drug discovery process
  • Save money, increase data quality in large genomics projects
Description Specifications
Drop volume 2.5 nL
Volume transfer range 2.5 nL – 10 µL (dependent on fluid type, assay, and source plate used)
Reagents supported DMSO, PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, antibodies, enzymes, and protein crystallography reagents
Transfer accuracy <10% deviation from target volume
Transfer precision <8% CV (except protein crystallography fluid class)
Source labware compatibility All Echo Qualified 384- and 1536-well Microplates
Destination labware compatibility All Echo Qualified Microplates and most ANSI-compliant/SBS-standard microplates in 96-, 384-, 1536-, and 3456-well formats 8 to 16 mm well height
Barcode reader options Short-side and/or long-side
Dimensions 53.9 cm width x 68.3 cm depth x 92.5 cm height (21.2 x 26.9 x 36.4 inches)
Weight 128 kg (283 lbs)
Working envelope Additional 25.4 cm (10 inches) on top, 2.5 cm (1 inch) on sides, 7.6 cm (3 inches) at front, 30.5 cm (12 inches) at back
Electrical AC 120V, 50/60 Hz, 10 A or AC 230V, 50/60Hz, 5 A
Operating temperature 21°C ± 5°C (70°F ± 9°F)
Vacuum House supply minimum 200 Torr (266 mbar, 22 inches Hg Vac)
OR
Standalone vacuum with surge tank, minimum pump speed 2.0 m3/hr, minimum pressure 100 Torr (133 mbar, 26 inches Hg Vac)
Fluid class calibration No calibration needed

Impacting Science

Acoustic liquid handling is changing how researchers are doing science. The precise nL transfer volumes, the any-well-to-any-well transfer capability, and flexible applications software of the Echo 550 Liquid Handler enables researchers to set up experiments not possible with traditional liquid handling.

Broad Range of Applications

With a combination of high transfer speeds and diverse fluid transfer capabilities, the Echo 550 Liquid Handler supports a wide range of applications in drug discovery and basic research.

GENOMICS
  • SANGER SEQUENCING ASSAY SET-UP
DRUG DISCOVERY
  • HIGH-THROUGHPUT SCREENING
  • HIGH CONTENT SCREENING
  • ASSAY-READY PLATES
  • EPIGENETICS
  • IC50

Key Benefits

  • Improved information about compound integrity and improved transfer quality through measurement of DMSO hydration level
  • Elimination of sample and compound loss, cross-contamination, carryover or leachates through the elimination of tips
  • Accurate, precise movement of nanoliter-scale quantities, enabling assay miniaturization, further reducing costs
  • Better results through direct dilution of compounds, an improvement over serial dilution methods
  • Greatly simplified assay development and dilution processes through the flexibility to transfer any volume of liquid from any well to any other well

VIDEO

The Echo Liquid Handling Technology

Echo Acoustic Liquid Handling|by Labcyte Inc.

The Echo Liquid Handler revolutionizes liquid handling with acoustic energy. Sound waves eject precisely sized droplets from a source onto a microplate, slide or other surface suspended above the source.

Decrease Cost, Maintain Data Quality

Fluorescence image reveals KYSE cells

The high performance fluid transfer of Echo 550 Liquid Handler delivers comparable transfer efficiency, knock-down, and cell morphology reactions to traditional liquid handlers with less use of sample and reagent.

FIGURE: Fluorescence image reveals KYSE cells successfully transfected with siGLO(GE Healthcare) (pink),transferred using the Echo Liquid Handler.

Inclusion intensities of esphogeal cancer cells transfected using the Echo Liquid Handler vs. manual transfer. Error bars indicate standard deviations.

Sample

  • ■ Echo Dispensed
  • ■ Manually Dispensed

Transition Into High-Throughput Formats

High assay performance at miniaturized volumes enables researchers to move quantitative assays into higher density formats. Assays such as time-dependent inhibition perform comparably to traditional volumes with less reagent, compound, and the elimination of workflow steps.

  • 1
  • 2
  • 3
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  • 5
  • - NADPH
  • + NADPH

FIGURE 1: Time-dependent CYP1A2 inhibition profiling with two inhibitors

EC50 (nM)
- NADPH 40.27
+ NADPH 7.96
δ IC50 5
  • 1
  • 2
  • 3
  • 4
  • 5
  • - NADPH
  • + NADPH

FIGURE 2: Time-dependent CYP1A2 inhibition profiling with two inhibitors

  EC50 (nM)
- NADPH 14273
+ NADPH 150.3
δ IC50 95