for high volume transfers
| Description | Specifications |
|---|---|
| Drop volume | 25 nL |
| Volume transfer range | 25 nL – 5 µL one well to one well |
| Transfer accuracy | <10% deviation from target volume |
| Transfer precision | <8% CV |
| Reagents supported | PCR and qPCR reagents, synthetic biology reagents, NGS reagents, primers and probes, cell culture media, proteins, nucleic acids, up to 50% glycerol, serum, plasma, and antibodies |
| Source labware compatibility | Echo Qualified 384 Polypropylene, 384 Polypropylene Plus, Reservoir, Microplates |
| Destination labware compatibility | All Echo Qualified Microplates and most ANSI-compliant/SBS-standard microplates in 96-, 384-well formats 8 -16 mm in height. Use of 1536-well plates as destination plates is application dependent |
| Dimensions | 53.9 cm width x 68.3 cm depth x 92.5 cm height (21.2 x 26.9 x 36.4 inches) |
| Weight | 128 kg (283 lbs) |
| Working envelope | Additional 25.4 cm (10 inches) on top, 2.5 cm (1 inch) on sides, 7.6 cm (3 inches) at front, 30.5 cm (12 inches) at back |
| Electrical | AC 120V, 50/60 Hz, 10 A or AC 230V, 50/60Hz, 5 A |
| Operating temperature | 21°C ± 5°C (70°F ± 9°F) |
| Vacuum | House supply minimum 200 Torr (266 mbar, 22 inches Hg Vac) OR Standalone vacuum with surge tank, minimum pump speed 2.0 m3/hr (70.68 cu ft/hr), minimum pressure 100 Torr (133 mbar, 26 inches Hg Vac) |
| Fluid class calibration | No calibration needed |
Introducing the Echo 525 Liquid Handler, the first rapid acoustic liquid handler designed specifically for biochemical and genomics reagent transfer. The Echo 525 Liquid Handler and the Echo Software Applications enable scientists to spend more time looking at data. Protocol creation and implementation in the Echo Software Applications are fast and does not require extensive training. Designed for use with the Echo System, the Access Laboratory Workstation expands the Echo 525 Liquid Handler’s utility and further frees up time.
The flexibility, speed, accuracy, and precision of the Echo 525 Liquid Handler enable researchers to build assays that are not possible with traditional liquid handlers. The use of acoustic sound energy removes the traditional boundaries of tip and pin tool based liquid handlers. Transfer variable fluid heights and viscosities from one source plate to enable rapid assay creation in one fluid transfer run. Say goodbye to fixed plate designs as the Echo Liquid Handler moves fluids from any well to any well enabling researchers to design the experimental plates they need to make new discoveries.
Assay assembly and the transfer of one reagent to many wells is simplified using the Echo Qualified Reservoir. With the capability of transferring up to 2.5 mL from each micro-well, you can build your assay without requiring an instrument change – saving time and money.
VIDEO
The Echo Liquid Handler revolutionizes liquid handling with acoustic energy. Sound waves eject precisely sized droplets from a source onto a microplate, slide or other surface suspended above the source.
The Echo 525 Liquid Handler transfers a wide range of fluids during one transfer including distilled water, buffers, nucleic acids, and reagents containing up to 50% glycerol. With its versatile fluid range, transfer volume range, any well to any well transfer capability, and powerful applications software the Echo 525 Liquid Handler supports a wide range of genomic assay workflows:
Sample transfer integrity is important for successful data generation. With highly precise and accurate non-contact transfers, the Echo 525 Liquid Handler introduces no cross-contamination during transfer of reagents or DNA.
FIGURE 1: G6PD amplification curves were generated using 600 nM G6PD primers, 300 nM G6Pd probes, 1X Roche RealTime ready DNA probes master mix, and 1 ng human cDNA. Results were read on a Roche LightCycler 480 system.
FIGURE 2: Source plate Echo Qualified 384-well Polypropylene Plus Microplate to a 384-well assay plate. 1 µL puc19 in 0.1 %TE per well was transferred. Roche 1536 DNA Green Master reagent following the standard protocol and data was read on a Roche Lightcycler 480.
| Volume | 5 µL | 7 µL | 10 µL |
|---|---|---|---|
| Cp Min | 23.46 | 23.57 | 22.59 |
| Cp Max | 25.69 | 24.68 | 23.22 |
| Cp Average | 24.21 | 24.08 | 22.90 |
| SD | 0.48 | 0.30 | 0.18 |
| CV | 1.99% | 1.23% | 0.81% |
Biochemical assays require the transfer of multiple fluid types during the experiment. The Echo 525 Liquid Handler successfully transfers antibodies, proteins, water, buffers, and highly viscous fluids from a single source plate making assay set up easy. Any well to any well transfer capability enables the creation of complex, multistep reagent addition and the ability to transfer nanoliter volumes enables successful miniaturization of complex assays.
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